KMID : 1144120150050020140
|
|
Biomedical Engineering Letters 2015 Volume.5 No. 2 p.140 ~ p.146
|
|
Fabrication of a cell-adhesive microwell array for 3-dimensional in vitro cell model
|
|
Park Ji-Hwang
Muller Michael Kim Jung-Tae Seidel Helmut
|
|
Abstract
|
|
|
Purpose: Microarrays of single living cells facilitate elucidation of the biological effects of a drug on a single cell providing large amounts of information regarding cellular chemistry at the molecular level in quantitative manner. Compared to 2D cultures, 3D in vitro cell models provide a more realistic cellular environment and permit the reproduction of in vitro cellular phenotypes.
Methods: We fabricated a microwell array for single cell analysis that could preserve the native morphology and functionality of HeLa cells by creating microwells with a cell-adhesive inner surface. Dopaminergic inorganic-organic hybrid resin (HR4-DOPA) was used for cell-adhesive layer and fabricated by spin-coating and micro contact printing methods. The physical dimensions of the microwells were designed to achieve high single-cell occupancy.
Results: Microwells was 25 ¡¿ 25 ¥ìm2 in size from the diameter of a HeLa cell. An optimal depth of microwells and cell concentration for HeLa single-cell occupancy was investigated; 25-¥ìm-deep microwells at 1.0 ¡¿ 106 cells/ml demonstrated the highest single-cell occupancy of 67.5% while providing a sufficient cell-adhesive surface area for long-term cell culture. We obtained singly occupied microwells with HeLa cells in 1072 microwells from 8 microwell arrays in the 2 ¡¿ 2mm2 area and with only 5.9% array-to-array variation.
Conclusions: The number of singly occupied microwells and array-to-array variation provides adequate throughput for accurate quantification in advanced single-cell analysis.
|
|
KEYWORD
|
|
Microwell array, 3-Dimensional in vitro cell model, Cell adhesive surface, Single cell occupancy, HeLa cell
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|